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Basic Info.
Product Description
Proteinase K (Lyophilizate, Ultra Pure)
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| C12100 | Proteinase K (Lyophilizate, Ultra Pure) | 1 g | $312.00 |
| C12101 | Proteinase K (Lyophilizate, Ultra Pure) | 10 g | $2,602.00 |
| C12102 | Proteinase K (Lyophilizate, Ultra Pure) | 100g | $23,410.00 |
| PDB-1000 | Buffer PDB | 1000ml | $300.00 |
△ Introduction
Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. It is not activated by metalions, chelating agents (for example, EDTA), sulfhydryl reagents, or by tripsinor chymotrypsin inhibitors. It is stable over a wide pH range (4-12.5), with optimal activity at pH 6.5-9.5. Activity can be stimulated by addition of denaturing agents (SDS and Urea). Rapid denaturation of the enzyme occurs at temperature above 70°C. Autolysis of the enzyme occurs increasingly at alkaline pH.
However, Proteinase K is not completely inactivated by autolysis. Some enzyme fragments continue to maintain their complete proteolytic activity, even after extensive autolysis.
Proteinase K is frequently used in molecular biology applications to digest unwanted proteins, such as nucleases in DNA or RNA preparations from microorganisms, cultured cells, and plants. The enzyme is typically used at 50-200 μg/ml in nucleic acid preparations at pH 7.5-8.0 and 37-55°C. Incubation times vary from 30 minutes to 18 hours.
△ Specifications
| Features | Specifications |
| Molecular Weight | 29.3kDa |
| Isoelectric Point | 8.9 |
| Appearance | White lyophilized powder |
| Purity | 95% (SDS-PAGE analysis) |
| Specific activity | ≥30 Units/mg protein |
| Temperature characteristic | The effective activity temperature is 37-70°C, and the enzyme activity at 65°C is twice that at 25°C. |
| PH characteristics | 4.0-12.0, the optimum range is ph7.5-11.5 |
| Preservation Conditions | It is recommended to store at -20°C to ensure the stability of activity to the greatest extent (normal temperature transportation or storage will not reduce enzyme activity). The shelf life is up to 3 years at -20°C and 2 years at 2-8°C. |
| Usage method | Prepare 20mg/ml with the solution, add proteinase K to the digestive solution or lysate until the final concentration is 50-200ug/ml, and incubate at 55-70°C. Proteinase K after reaction can be removed or inactivated by magnetic bead method, column method or phe nol chloro form extraction. Protease K can be inactivated by incubation at 95°C for 3 minutes or 70°C for 15 minutes. |
| Nucleic acid residue detection | Qubit did not detect Human DNA contamination not detected (real time PCR) Note: 1g protease K is used for nucleic acidextraction and eluted by 40ul Elution Buffer. |
| Nuclease detection | DNase not detected RNase not detected |
| Recommended application | Nucleic acid extraction, virus nucleic acid extraction |
△ Advantages
- Not including RNase, DNase and Nickase
- No nucleic acid residue (not detected by Qubit after extracting the whole 100mg protein K)
- No human-source DNA contamination (not detected by Q-PCR)
- No bacterial DNA contamination (not detected by 16S universal primer)
- No fungal DNA contamination (not detected by 1TS universal primer)
- Can be transported and valid for two years at normal temperature in dry condition.
- Can be used for highly sensitive free DNA extraction, virus nucleic acid extraction, whole blood DNA extraction, etc.
△ Ordering information
| Contents | C12100 | C12101 | C12102 | PDB-1000 |
| Proteinase K, Lyophilizate, >30 units/mg of protein | 1 g | 10 g | 100 g | |
| Buffer PDB (20mM Tris, pH 7.5, 10mM CaCl2, 50% glycerol, 0.1% Preservatives) | 1000 ml |
